Evaluation of confocal microscopy system performance
نویسندگان
چکیده
منابع مشابه
Evaluation of confocal microscopy system performance.
BACKGROUND The confocal laser scanning microscope (CLSM) has been used by scientists to visualize three-dimensional (3D) biological samples. Although this system involves lasers, electronics, optics, and microscopes, there are few published tests that can be used to assess the performance of this equipment. Usually the CLSM is assessed by subjectively evaluating a biological/histological test s...
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BACKGROUND The coefficient of variation (CV) is defined as the standard deviation (sigma) of the fluorescent intensity of a population of beads or pixels expressed as a proportion or percentage of the mean (mu) intensity (CV = sigma/mu). The field of flow cytometry has used the CV of a population of bead intensities to determine if the flow cytometer is aligned correctly and performing properly...
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متن کاملConfocal Microscopy
A confocal microscope creates sharp images of a specimen that would otherwise appear blurred when viewed with a conventional microscope. This is achieved by excluding most of the light from the specimen that is not from the microscope’s focal plane. The image has less haze and better contrast than that of a conventional microscope and represents a thin cross-section of the specimen. Thus, apart...
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ژورنال
عنوان ژورنال: Cytometry
سال: 2001
ISSN: 0196-4763,1097-0320
DOI: 10.1002/1097-0320(20010801)44:4<273::aid-cyto1120>3.0.co;2-n